|
KMID : 1007520110200020519
|
|
Food Science and Biotechnology
2011 Volume.20 No. 2 p.519 ~ p.524
|
|
|
Effect of Mung Bean Ethanol Extract on Pro-inflammtory Cytokines in LPS Stimulated Macrophages
|
|
Lee Suk-Jun
Lee Ji-Hye
Lee Han-Hyung
Lee Seul
Kim Sae-Hun
Chun Tae-Hoon
Imm Jee-Young
|
|
|
Abstract
|
|
|
|
The anti-inflammatory effect of mung bean ethanol extract on lipopolysaccharide (LPS) stimulated macrophages (J774) was evaluated. The mung bean extract was separated into 5 fractions by normal phase silica gel column chromatography and the mRNA expressions of pro-inflammatory cytokines were examined after incubation with each fraction in LPS stimulated macrophages. All pro-inflammatory cytokines including interleukin (IL)-1β, IL-6, IL-12β, tumor necrosis factor (TNF)-α, and inducible NO synthase (iNOS) were remarkably decreased in the cells treated with 3.7 mg/mL of F3 fraction. The active F3 fraction did not show any cytotoxcity according to propidium iodide staining and no inhibitory effects on J774 cell proliferation were observed by MTT assay. The active fraction contained gallic acid, vitexin, and isovitexin as major components.
|
|
|
KEYWORD
|
|
|
mung bean ethanol extract, inflammation, macrophage, pro-inflammatory cytokine
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|